测量荧光偏振
为了评估极化,需要两个发射测量:第一个使用平行于激发滤光片(S平面)的偏振发射滤光片,第二个使用垂直于激发滤光片(P平面)的偏振发射滤光片。 典型的标记染料是FITC,BODIPY或TMR(TRITC)。
有关荧光偏振的应用集
Interaction between ER alpha and SCR-1 NR-2 through FP with Mithras Characterization of the interaction between recombinant estrogen receptor alpha and transcriptional coactivator SCR-1 NR-2 binding motif through Fluorescence Polarization study with Mithras LB 940
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