Brochure of the Centro and Centro XS microplate luminometers
PDF | 461.4 KB
即可下载Centro支持所有基本的发光技术,包括:
由于对在光子计数模式下工作的光电倍增管的严格筛选,以及40多年的发光计制造经验,Centro 提供了高度灵敏的发光检测功能:可以可靠地检测到低于5 amol ATP /孔(25 fM)的水平。 在Centro XS中,对光学器件进行了优化,以进一步提高其灵敏度:低于1.8 amol(9 fM)/孔。
运行样品时,您是否经常看到一些高强度样品被低强度孔包围? 然后,您可能会受到称为交叉干扰的现象的影响,这种现象可能会产生错误的结果,从而导致错误的结论。 Centro的降低孔间干扰的设计几乎消除了这个问题:孔间干扰为10-6时,您不必担心高强度样品在板上的位置。
借助其内置的废液泵,您无需担心装载微孔板,废液容器或卸下用于清洗和灌注试剂管道的进样针头的麻烦; 内置的废液泵负责安全清除多余的液体。
Centro 最多可配备3个喷注式进样器,这些进样器在预检位或检测位置具有可变的体积。 喷注式进样器的超快进样速度消除了进样和测量之间的任何延迟,并能够测量快速反应,例如: 闪光发光。
伯托公司的喷注式进样器使用聚四氟乙烯波纹管精确和快速进样,并保证最有效的混合和极长的寿命。
**Centro XS 中所有的进样器都是在预检位。
我们的微孔板读数器由LightCompass®驱动,这是一个灵活的软件,可以使用勃透科技的仪器轻松地进行数据采集、分析和报告。它已经针对不同的微孔板和试管格式进行了优化。LightCompass®的特点是
你可以从一个先进的科学软件中期待所有的功能,如灵活创建测量
曲线拟合、定性分析以及符合FDA 21 CFR Part 11的规定。
LightCompass®有3个不同的版本: 基本版、加强版和专业版。只要选择更适合你的研究需要的版本即可。
检测器 | 单光子计数模式的低噪声光电倍增管 |
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检测技术 | 发光 |
测量模式 | 终点、动力学、重复、区域扫描(每孔高达100 x 100点) |
发光光谱范围 | 340 - 630nm |
进样器 | 多达3个喷注式进样器,无摩擦操作 |
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进样器体积 | 10-100µL ,步进1µL |
进样器的准确性 | 优于2% |
进样器的精度 | 优于2% |
微孔板类型 | 96孔和384*孔 |
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*仅限Centro XS
振荡 | 线性,轨道,双轨道,速度和直径可选择 |
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温度控制 | 从室温+5°C到45°C(Centro XS无温控模块) |
发光的敏感性 | Centro: <5 amol (25 fM) ATP/well(96孔板) Centro XS: <1.8 amol (7.5 fM) ATP/well(96孔板) |
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孔间干扰 | 10-6 |
动态范围 | >6个数量级 |
接口 | Windows操作系统,通过USB连接 |
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操作系统 | Windows 10 |
可选软件包 | LightCompass®:用于数据采集、分析和报告的灵活软件 |
规则 | CE, NRTL |
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电源 | 100 – 240 VAC ±10%, 50/60 Hz |
操作电压 | 24 VDC / 6.67 A |
温度范围 | 储存: 0-40° C 操作: 15-35° C |
湿度范围 | 10-80% 无凝结 温度高达31°C时最大相对湿度为80%; 最高40°C时相对湿度 线性降低至50% |
尺寸 (W x D x H) | 334 x 437 x 287 mm |
重量 | 21.8 kg |
Brochure of the Centro and Centro XS microplate luminometers
PDF | 461.4 KB
即可下载Mycoplasma is a common contaminant in cell cultures which can compromise the…
EN | PDF | 361.9 KB
即可下载Validation of the Centro LB 963 for the Dual-Luciferase® Reporter System from…
EN | PDF | 300.4 KB
即可下载Brochure of the Centro and Centro XS microplate luminometers
PDF | 461.4 KB
即可下载Mycoplasma is a common contaminant in cell cultures which can compromise the…
EN | PDF | 361.9 KB
即可下载Validation of the Centro LB 963 for the Dual-Luciferase® Reporter System from…
EN | PDF | 300.4 KB
即可下载在科学文献中,有很多使用Centro的文章。在这里,你可以选择最近的论文。
Year | Authors | Journal | Title | Method |
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2023 | Špela Tomaž, Marko Petek, Tjaša Lukan, Karmen Pogačar, Katja Stare, Erica Teixeira Prates, Daniel A Jacobson, Jan Zrimec, Gregor Bajc, Matej Butala, Maruša Pompe Novak, Quentin Dudley, Nicola Patron, Ajda Taler-Verčič, Aleksandra Usenik, Dušan Turk, Salomé Prat, Anna Coll, and Kristina Gruden | Plant Physiology | In planta luciferase transactivation assay | |
2022 | Sarah Temmam, Khamsing Vongphayloth, Eduard Baquero, Sandie Munier, Massimiliano Bonomi, Béatrice Regnault, Bounsavane Douangboubpha, Yasaman Karami, Delphine Chrétien, Daosavanh Sanamxay, Vilakhan Xayaphet, Phetphoumin Paphaphanh, Vincent Lacoste, Somphavanh Somlor, Khaithong Lakeomany, Nothasin Phommavanh, Philippe Pérot, Océane Dehan, Faustine Amara, Flora Donati, Thomas Bigot, Michael Nilges, Félix A. Rey, Sylvie van der Werf, Paul T. Brey & Marc Eloit | Nature | Bat coronaviruses related to SARS-CoV-2 and infectious for human cells | Pseudovirus assays (firefly luciferase) |
2022 | Jianyuan Zhao, Qian Liu,Dongrong Yi, Quanjie Li, SaiSai Guo, Ling Ma, Yongxin Zhang, Dongxin Dong, Fei Guo, Zhenlong Liu, Tao Wei, Xiaoyu Li, and Shan Cena | Antiviral Research | Gaussia Luciferase | |
2021 | Hannah Kinoshita, Jessica Durkee-Shock, Mariah Jensen-Wachspress, Vaishnavi V. Kankate, Haili Lang, Christopher A. Lazarski, Anjeni Keswani, Kathleen C. Webber, Kimberly Montgomery-Recht, Magdalena Walkiewicz, Luigi D. Notarangelo, Peter D. Burbelo, Ivan Fuss, Jeffrey I. Cohen, Catherine M. Bollard, and Michael D. Keller | Journal of Clinical Immunology | Robust Antibody and T Cell Responses to SARS-CoV-2 in Patients with Antibody Deficiency | Luciferase immunoprecipitation |
2021 | Chao Yang, Rui Liu, Jinhuan Pang, Bin Ren, Huanbin Zhou, Gang Wang, Ertao Wang, and Jun Liu | Nature Communications | Oxidative burst and MAPK | |
2021 | Alexandra Rhoden, Felix W. Friedrich, Theresa Brandt, Janice Raabe, Michaela Schweizer, Jana Meisterknecht, Ilka Wittig, Bärbel M. Ulmer, Birgit Klampe, June Uebeler, Angelika Piasecki, Kristina Lorenz, Thomas Eschenhagen, Arne Hansen, and Friederike Cuello | Redox Biology | Lactate-Glo™ | |
2021 | Chunyan Wang, Rien van Haperen, Javier Gutiérrez-Álvarez, Wentao Li, Nisreen M. A. Okba, Irina Albulescu, Ivy Widjaja, Brenda van Dieren, Raul Fernandez-Delgado, Isabel Sola, Daniel L. Hurdiss, Olalekan Daramola, Frank Grosveld, Frank J. M. van Kuppeveld, Bart L. Haagmans, Luis Enjuanes, Dubravka Drabek, and Berend-Jan Bosch | Nature Communications | Pseudotyped virus neutralization assay | |
2021 | Emeline Bonsergent, Eleonora Grisard, Julian Buchrieser, Olivier Schwartz, Clotilde Théry, and Grégory Lavieu | Nature Communications | Extracellular vesicle uptake using NLuc | |
2021 | Silke D. Werle, Julian D. Schwab, Marina Tatura, Sandra Kirchhoff, Robin Szekely, Ramona Diels, Nensi Ikonomi, Bence Sipos, Jan Sperveslage, Thomas M. Gress, Malte Buchholz, and Hans A. Kestler | Cancers | Unraveling the Molecular Tumor-Promoting Regulation of Cofilin-1 in Pancreatic Cancer | Luminescent BrdU assay |
2021 | Seiya Ozono, Yanzhao Zhang, Hirotaka Ode, Kaori Sano, Toong Seng Tan, Kazuo Imai,Kazuyasu Miyoshi, Satoshi Kishigami, Takamasa Ueno, Yasumasa Iwatani, Tadaki Suzuki & Kenzo Tokunaga | Nature Communications | SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity | Pseudotyped virus quantification using Nano-Glo® HiBiT Lytic Detection System |
2020 | Maureen Spit, Nicola Fenderico, Ingrid Jordens, Tomasz Radaszkiewicz,Rik GH Lindeboom, Jeroen M Bugter, Alba Cristobal, Lars Ootes, Max van Osch,Eline Janssen, Kim E Boonekamp, Katerina Hanakova, David Potesil, Zbynek Zdrahal,Sylvia F Boj, Jan Paul Medema, Vitezslav Bryja, Bon-Kyoung Koo, Michiel Vermeulen & Madelon M Maurice | The EMBO Journal | RNF43 truncations trap CK1 to drive niche-independent self-renewal in cancer | Dual Luciferase |
2020 | Alexandre Quilbe, Olivier Moralès, Martha Baydoun, Abhishek Kumar, Rami Mustapha, Takashi Murakami, Bertrand Leroux, Clémentine de Schutter, Elise Thecua, Laurine Ziane, Ludovic Colombeau, Céline Frochot, Serge Mordon, and Nadira Delhem | Journal of Clinical Medicine | An Efficient Photodynamic Therapy Treatment for Human Pancreatic Adenocarcinoma | CellTiter-Glo® |
2020 | Sungmi Song, Jin-Young Lee, Ludmila Ermolenko, Aloran Mazumder, Seungwon Ji, Heeju Ryu, HyeJin Kim, Dong-Wook Kim, Jung Weon Lee, Mario Dicato, Christo Christov, Michael Schnekenburger, Claudia Cerella, Déborah Gérard, Barbora Orlikova-Boyer, Ali Al-Mourabit and Marc Diederich | International Journal of Molecular Sciences | GSH/GSSG-Glo™ | |
2020 | Urooj Fatima, Zhenyu Zhang, Haili Zhang, Xue-Feng Wang, Ling Xu, Xiaoyu Chu,Shuang Ji and Xiaojun Wang | Viruses | Dual Luciferase | |
2020 | Stefan Reich, Chi D. L. Nguyen, Canan Has, Sascha Steltgens, Himanshu Soni, Cristina Coman,Moritz Freyberg, Anna Bichler, Nicole Seifert, Dominik Conrad, Christiane B. Knobbe-Thomsen,Björn Tews, Grischa Toedt, Robert Ahrends & Jan Medenbach | Nature Communications | NADP/NADPH-Glo™ | |
2020 | Yeojung Nam, Kyung Min Kang, Se Ra Sung, Ji Eun Park, Yun-Jeong Shin, Seung Hun Song, Ju Tae Seo, Tae Ki Yoon, Sung Han Shim | Asian Journal of Andrology | Dual Luciferase | |
2020 | Monica Z. Wu, Haruichi Asahara, George Tzertzinis, and Bijoyita Roy | RNA | Synthesis of low immunogenicity RNA with high-temperature in vitro transcription | BioLux® Cypridina Luciferase Assay |
2020 | Da Hee Lee, Na Eun Kwon, Won-Ji Lee, Moo-Seung Lee, Doo-Jin Kim, Ji Hyung Kim and Sung-Kyun Park | Cells | Increased O-GlcNAcylation of c-Myc Promotes Pre-BCell Proliferation | Glucose Uptake-Glo™ |
2020 | Qingkang Lyu, Irene S. Ludwig, Peter J. S. Kooten, Alice J. A. M. Sijts, Victor P. M. G. Rutten, Willem van Eden, Femke Broere | Cell Stress and Chaperones | Steady-Glo® | |
2020 | Daichi Hayashi, Toshiharu Shirai, Ryu Terauchi, Shinji Tsuchida, Naoki Mizoshiri, Yuki Mori, Yuji Arai, Osam Mazda, and Toshikazu Kubo | Oncology Letters | Pristimerin inhibits the proliferation of HT1080 fibrosarcoma cells by inducing apoptosis | RealTime-Glo™ MT Cell Viability Assay |
2020 | Stefano Del Prato, Jahoon Kang, Michael E. Trautmann, John Stewart, Christopher H. Sorli, Michael Derwahl, Alfonso Soto and Kun‐Ho Yoon | Diabetes, Obesity and Metabolism | cAMP-Glo™ | |
2020 | Vito Lampasona, Massimiliano Secchi, Marina Scavini, Elena Bazzigaluppi, Cristina Brigatti, Ilaria Marzinotto, Alberto Davalli, Amelia Caretto, Andrea Laurenzi, Sabina Martinenghi, Chiara Molinari, Giordano Vitali, Luigi Di Filippo, Alessia Mercalli, Raffaella Melzi, Cristina Tresoldi, Patrizia Rovere-Querini, Giovanni Landoni, Fabio Ciceri, Emanuele Bosi and Lorenzo Piemonti | Diabetologia | LIPS with NanoLuc® Luciferase | |
2019 | Jun Weng, Xu Han, Kaiyu Liu, Jiong Yang, Shiruo Wei, Yue Zhang, Fanhong Zeng, Yang Li, Li Shen, Yi Gao | International Journal of Biological Sciences | Dual-Glo® |
您可以在出版物数据库中找到使用Centro仪器的其他出版物。
请记住,所提供的清单并不详尽,但它提供了用我们的仪器进行实验并在同行评议的杂志上发表的宝贵例子。